EDUCATION

2005.8                Ph. D. in Physical Chemistry, Seoul National University

Thesis: “Single-molecule Fluorescence Resonance Energy Transfer by Alternating-Laser Excitation”

2000.2                M. Sc. in Physical Chemistry, Seoul National University

1998.2                B.S. in Chemistry, Seoul National University

ACADEMIC POSITIONS

2017.3-               Associate Professor, Department of Chemistry, Seoul National University

2013.9-2017.2     Associate Professor, Department of Physics and I-Bio, POSTECH

2009.1-2013.8     Assistant Professor, I-Bio and Department of Physics, POSTECH

2006.5-2008.12  Postdoctoral Fellow, Harvard University (Advisor: Prof. X. Sunney Xie)

2005.9-2006.4    Postdoctoral Fellow, Seoul National University (Advisor: Prof. S. K. Kim)

2002.2-2003.8   Visiting scholar, University of California at Los Angeles (Advisor: Prof. Shimon Weiss)

AWARDS

2005     The Korean Chemical Society Award for Outstanding Ph.D. Thesis

2014     Advisor of the recipient of Samsung HumanTech paper award (Gold Medal in Basic Science & Technology)
　

RESEARCH AREAS

- Methods : Single-molecule Fluorescence and FRET, Single Fluorescent Protein imaging in living cell, Super-resolution optical imaging (PALM), Single cell analysis on microfluidic device

- Biology : Signal transduction by EGFR and GPCR, Membrane protein dynamics, Transcriptional dynamics, DNA recombination, Neurotransmitter release, Parkinson’s and Alzheimer’s disease
　

REPRESENTATIVE PUBLICATIONS (* corresponding author)

1.     C. Kim, O. Lee, J.-Y. Kim, W. Sung, N. K. Lee*, "Dynamic Release of Bending Stress in Short dsDNA by Formation of a Kink and Forks", Angew. Chem. Int. Ed. 54, 8943-8947 (2015).

2.      J.-Y. Kim, C. Kim, N. K. Lee,* “Real-time submillisecond single-molecule FRET dynamics of freely diffusing molecules with liposome tethering”, Nature Communications 6, 6992 (2015).

3.     D.-H. Kim^#, K. Zhou^#, D.-K. Kim^#, S. Park, J. Noh, Y. Kwon, D. Kim, N. W. Song, J.-B. Lee, P.-G. Suh, N. K. Lee*, S. H. Ryu,* “Analysis of Interactions between the Epidermal Growth Factor Receptor and Soluble Ligands on the Basis of Single-Molecule Diffusivity in the Membrane of Living Cells”, Angew. Chem. Int. Ed. 24, 7028–7032 (2015).

4.     S. Yang, S. Kim, Y. R. Lim, C. Kim, H. J. An, J-H. Kim, J. Sung*, N. K. Lee,* “Contribution of RNA polymerase concentration variation to protein expression noise”, Nature Communications 5, 4761 (2014).

5.      B. K. Choi^#, M.-G. Choi^#, J.-Y. Kim,^# Y. Yang, Y. Lai, D.-H. Kweon, N. K. Lee,* Y.-K. Shin,* “Large α-Synuclein oligomers inhibit neuronal SNARE-mediated vesicle docking”, Proc. Natl. Acad. Sci. USA 110, 4087–4092 (2013).

6.      J.Y. Kim^#, B. K Choi^#, M. G Choi, S. A. Kim, Y. K. Shin,* N. K. Lee,* “Solution single-vesicle assay reveals PIP2 is required for the sequential actions of synaptotagmin-1 on SNAREs”, EMBO J. 31, 2144-2155 (2012).

7.      N. K. Lee, H. R. Koh, K. Y. Han, and S. K. Kim, “Folding of 8-17 deoxyribozyme studied by three-color alternating-laser excitation of single-molecules”, J. Am. Chem. Soc. 129, 15526 (2007).

8.     N. K. Lee, A. N. Kapanidis, H. R. Koh, Y. Korlann, S. O. Ho, N. Gassman, S. K. Kim, and S. Weiss, “Three-Color Alternating-Laser Excitation of Single Molecules: Monitoring Multiple Interactions and Distances”, Biophys. J. 92, 303 (2007). 103 times cited

9.    N. K. Lee^#, A. N. Kapanidis^#, Y. Wang, X. Michalet, J. Mukhopadhyay, R. H. Ebright, and S. Weiss, “Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitation” Biophys. J. 88, 2939 (2005). #equal contribution. 179 times cited

10.    A. N. Kapanidis^#, N. K. Lee^#, E. Margeat, T. Laurence, S. Doose, and S. Weiss, “Fluorescence-Aided Molecule Sorting: analysis of structure and interactions by alternating-laser excitation of single molecules”, Proc. Natl. Acad. Sci. USA 101, 8936 (2004). #equal contribution. 259 times cited